BRG-1 antibody (mAb)

for BRG-1 antibody (mAb) (Clone 3G4)Background

BRG1 (Brahma-related gene-1, SNF2β) is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. BRG1 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian BRG1 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. BRG1 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency.

for BRG-1 antibody (mAb) (Clone 3G4)Immunogen

This BRG-1 antibody was raised against a recombinant protein corresponding to amino acids 214-279 of mouse BRG1.

for BRG-1 antibody (mAb) (Clone 3G4)Application Notes

This BRG-1 antibody has been validated for use in immunofluorescence (IF, 1 µg per ml dilution) and Western blot (0.5 - 2 µg per ml dilution).

for BRG-1 antibody (mAb) (Clone 3G4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for BRG-1 antibody (mAb) (Clone 3G4)References

This antibody was used in immunofluorescence and Western blot as described below:
Ohkawa, Y. et al (2009) Hybridoma 28: 463-466.

for BRG-1 antibody (mAb) (Clone 3G4)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for BRG-1 antibody (mAb) (Clone 3G4)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.