Sp1 non-phospho antibody

for Sp1 non-phospho antibodyBackground

Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The Sp1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain.

Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair.

for Sp1 non-phospho antibodyImmunogen

This Sp1 antibody was raised against a peptide containing serine 101 of human Sp1.

for Sp1 non-phospho antibodyApplication Notes

This Sp1 antibody has been validated for use in Western blot (1:500 - 1:1,000 dilution).

This antibody recognizes Sp1 when it is not phosphorylated at serine 101.

for Sp1 non-phospho antibodyBuffer

PBS pH 7.5, 30% glycerol, 0.035% sodium azide.

for Sp1 non-phospho antibodyStorage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Sp1 non-phospho antibodyGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.