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Dicer antibody (mAb)
| Clone: N167/7 |
| Catalog No: 39817 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39818 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | — |
for Dicer antibody (mAb) (Clone N167/7)Background
Dicer is a double-stranded RNase and part of the micro-RNA (miRNA) loading complex (miRLC), which is composed of Dicer, Ago2 and TARBP2. Dicer is required for formation of the RNA induced silencing complex (RISC). Dicer and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto Ago2. Dicer also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the destruction of complementary RNAs.
for Dicer antibody (mAb) (Clone N167/7)Immunogen
This Dicer antibody was raised against a recombinant protein corresponding to amino acids 1638-1899 of mouse Dicer.
for Dicer antibody (mAb) (Clone N167/7)Application Notes
This Dicer antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution) and immunocytochemistry.
for Dicer antibody (mAb) (Clone N167/7)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Dicer antibody (mAb) tested by Western blot.
Cytoplasmic extract of mouse ES cells (40 µg per lane) probed with Dicer antibody (2 µg/ml).
for Dicer antibody (mAb) (Clone N167/7)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Dicer antibody (mAb) (Clone N167/7)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
