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Antibodies

MPP8 antibody (mAb)

Clone: N119/44
Catalog No: 39819 Format: 100 µg $340 Buy Now
Catalog No: 39820 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Applications:ICC, WB
Reactivity:Human
Molecular Weight:120 kDa

Applications Table

Western Blot Chromatin IP
0.5 - 2 µg/ml

for MPP8 antibody mAb (Clone N119/44)Background

MPP8 (M-Phase Phosphoprotein 8) was originally identified as a phosphoprotein that reacts with MPM2, an antibody that labels mitosis-specific and cell cycle-regulated phosphoproteins. MPP8 contains a single chromodomain that preferentially interacts with nucleosomes enriched for histone H3 lysine 9 methylation but not lysine 4 methylation.

for MPP8 antibody mAb (Clone N119/44)Immunogen

This MPP8 antibody was raised against a recombinant protein corresponding to amino acids 50-120 of human MPP8 (M-phase phosphoprotein 8).

for MPP8 antibody mAb (Clone N119/44)Application Notes

This MPP8 antibody has been validated for use in Western blotting (0.5 - 2 µg/ml dilution) and immunocytochemistry.

for MPP8 antibody mAb (Clone N119/44)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

 
MPP8 antibody tested by Western blot.

MPP8 antibody (mAb) tested by Western blot.
Nuclear extract of HEK293 cells (20 µg per lane) probed with MPP8 antibody (mAb) (2 µg/ml dilution).

for MPP8 antibody mAb (Clone N119/44)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MPP8 antibody mAb (Clone N119/44)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

MPP8 antibody (mAb)
 

Data Thumbnails

MPP8 antibody tested by Western blot.

Western blot of MPP8 antibody (mAb).
(Click image to enlarge and see details).