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Sox2 antibody (pAb)
| Catalog No: 39823 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39824 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 1:2,000 - 1:10,000 | 2 - 10 µl per ChIP | 1:2,000 - 1:4,000 | 2 - 10 µl per IP |
for Sox2 antibody (pAb)Background
Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins. SOX proteins have an HMG box that binds DNA. Sox2 forms a complex with Oct4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and for embryonic stem cell pluripotency and thus can serve as a stem cell marker. Overexpression of Sox2 (along with Oct4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndromic type 3.
for Sox2 antibody (pAb)Immunogen
This Sox2 antibody was raised against a peptide corresponding to amino acids amino acids 111-140 mouse Sox2.
for Sox2 antibody (pAb)Application Notes
This Sox2 antibody has been validated for use in chromatin immunoporecipitation (ChIP, 2 - 10 µl per ChIP), immunofluorescence (IF, 1:2,000 - 1:4,000), immunohistochemistry, immunoprecipitation (IP, 2 - 10 µl per IP) and Western blotting (1:2,000 - 1:10,000).
See reference Tompkins, et al 2009 for more details.
for Sox2 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Sox2 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Sox2 antibody (pAb).
for Sox2 antibody (pAb)References
This antibody was used as described in Tompkins, D.H. et al (2009) PLoS One 4: e8248.
Sox2 antibody (pAb) tested by Western blot.
HeLa cell extract expressing mouse Sox2 (50 µg per lane) probed with Sox2 antibody at a dilution of 1:5,000.
for Sox2 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Sox2 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
