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MLL1/HRX antibody (mAb)
| Clone: N4.4 |
| Catalog No: 39829 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39830 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 0.5 - 2 µg/ml | Published. See Application Notes below. |
Published. See Application Notes below. |
for MLL1/HRX antibody (mAb) (Clone N4.4)Background
MLL1 (Mixed-lineage leukemia protein 1, KMT2A, TRX1, HRX1, ALL1) is a SET-domain containing histone methyltransferase specific for lysine 4 of of histone H3. It serves as a transcriptional activator for many target genes, including several HOX genes. MLL is a frequent target for translocations in acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL) and mixed lineage leukemia (MLL). A key feature of MLL fusion proteins is their ability to efficiently transform hematopoietic cells into leukemia stem cells. Leukemogenic MLL translocations encode MLL fusion proteins that have lost H3 Lys4 methyltransferase activity. MLL1 is found in a complex of proteins that includes ASH2L, WDR5, DPY30 and RBBP5.
for MLL1/HRX antibody (mAb) (Clone N4.4)Immunogen
This MLL1 antibody was raised against a recombinant protein corresponding to amino acids 161-356 of mouse MLL1.
for MLL1/HRX antibody (mAb) (Clone N4.4)Application Notes
This MLL1 antibody has been validated for use in chromatin immunoprecipitation (ChIP), immunoprecipitation (IP) and Western blotting (0.5 - 2 µg/ml).
See references for more information on specific applications.
for MLL1/HRX antibody (mAb) (Clone N4.4)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with MLL1/HRX antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MLL1/HRX antibody (mAb).
for MLL1/HRX antibody (mAb) (Clone N4.4)References
This antibody was used as described in
ChIP: Jin, S. et al (2010) J Clin Invest 281: 593-606.
IP: Yokoyama, A. et al (2009) Blood 100: 3710-3718.
WB: Yokoyama, A. et al (2009) Blood 100: 3710-3718.
for MLL1/HRX antibody (mAb) (Clone N4.4)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for MLL1/HRX antibody (mAb) (Clone N4.4)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
