HNF-3α / FOXA1 antibody (mAb)

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)Background

HNF-3α (Hepatocyte nuclear factor 3 alpha, forkhead box A1, FoxA1) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1α, HNF-1β, HNF-3α, HNF-3β, HNF-3γ, HNF-4α, HNF-4γ, HNF-6α and HNF-6β), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3α and HNF-3γ have also been shown to be involved in gastrulation events such as body axis formation.

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)Immunogen

This HNF-3α / FOXA1 antibody was raised against a recombinant protein corresponding to amino acids 7-103 of rat HNF-3α /FOXA1.

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)Application Notes

This HNF-3α / FOXA2 antibody has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution) and immunohistochemistry (1:2,000; see references).

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)Positive Control

Active Motif's Rat Liver nuclear extract (Catalog No. 36024) can be used as a positive control for HNF-3α antibody.

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)References

This antibody has been used as described in the following references:
IHC: Albergaria A et al (2009) Breast Cancer Res 11: R40
IHC: Nucera C et al (2009) Clin Cancer Res 15: 3680-3689

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HNF-3α / FOXA1 antibody mAb (Clone 2F83)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.