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HNF-3α / FOXA1 antibody (mAb)

Clone: 2F83
Aliases: TCF3A
商品コード: 39837 Format: 100 µg ¥69,000 Buy Now
商品コード: 39838 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:50 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunohistochemistry Validated

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)Application Notes


Validated Applications:
ChIP: 5 - 10 µg per ChIP
ChIP-Seq: 5 - 10 µg each
WB: 1 - 5 µg/ml dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Published Applications:
IHC
WB
See references for more information. Individual optimization may be required.

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)Immunogen

This HNF-3α / FOXA1 antibody was raised against a recombinant protein corresponding to amino acids 7-103 of rat HNF-3α /FOXA1.

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)References

 
HNF-3α / FOXA1 antibody (mAb) tested by ChIP-Seq.

HNF-3α / FOXA1 antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from LnCaP cells and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HNF-3α / FOXA1 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

HNF-3α / FOXA1 antibody (mAb) tested by Western blot.

HNF-3α / FOXA1 antibody (mAb) tested by Western blot.
Extract of HeLa cells over-expressing mouse HNF-3α / FOXA1 (50 µg per lane) probed with HNF-3β / FOXA1 antibody (mAb) (1:5,000 dilution).

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)Background

HNF-3α (Hepatocyte nuclear factor 3 alpha, forkhead box A1, FoxA1) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1α, HNF-1β, HNF-3α, HNF-3β, HNF-3γ, HNF-4α, HNF-4γ, HNF-6α and HNF-6β), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3α and HNF-3γ have also been shown to be involved in gastrulation events such as body axis formation.

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)Positive Control

Active Motif's Rat Liver nuclear extract (Catalog No. 36024) can be used as a positive control for HNF-3α antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with HNF-3α / FOXA1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use HNF-3α / FOXA1 antibody (mAb).

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HNF-3α / FOXA1 antibody (mAb)
 

Data Thumbnails

HNF-3α / FOXA1 antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of HNF-3α / FOXA1 mAb.
(Click image to enlarge and see details.)

HNF-3α / FOXA1 antibody (mAb) tested by Western blot.

Western blot of HNF-3α / FOXA1 mAb.
(Click image to enlarge and see details.)