HNF-3α / FOXA1 antibody (mAb)

RRID: AB_2793362
Clone: 2F83
Aliases: TCF3A
Catalog No: 39837 Format: 100 µg ¥88,000 Buy
Catalog No: 39838 Format: 10 µg ¥24,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
50 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunohistochemistry Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 5 - 10 µg per ChIP
ChIP-Seq: 5 - 10 µg each
WB: 1 - 5 µg/ml dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.

This product is currently on indefinite backorder.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IHC(P)
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This HNF-3α / FOXA1 antibody was raised against a recombinant protein corresponding to amino acids 7-103 of rat HNF-3α /FOXA1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

HNF-3α / FOXA1 antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from LnCaP cells and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HNF-3α / FOXA1 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

HNF-3α / FOXA1 antibody (mAb) tested by Western blot.
Extract of HeLa cells over-expressing mouse HNF-3α / FOXA1 (50 µg per lane) probed with HNF-3β / FOXA1 antibody (mAb) (1:5,000 dilution).

Background

HNF-3α (Hepatocyte nuclear factor 3 alpha, forkhead box A1, FoxA1) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1α, HNF-1β, HNF-3α, HNF-3β, HNF-3γ, HNF-4α, HNF-4γ, HNF-6α and HNF-6β), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3α and HNF-3γ have also been shown to be involved in gastrulation events such as body axis formation.

Positive Control

Active Motif's Rat Liver nuclear extract (Catalog No. 36024) can be used as a positive control for HNF-3α antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with HNF-3α / FOXA1 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting