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SIN3A antibody (pAb)

Aliases: Switch independent 3 homologue
商品コード: 39865 Format: 100 µl ¥67,000 Buy Now
商品コード: 39866 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:155 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated

for SIN3A antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µl per ChIP
WB: 1:1,000 - 1:5,000 dilution

for SIN3A antibody (pAb)Immunogen

This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A.

for SIN3A antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SIN3A antibody (pAb)References

 
SIN3A antibody (pAb) tested by ChIP.

SIN3A antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from TM4 Sertoli cells and 5 µl of SIN3A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

SIN3A antibody (pAb) tested by Western blot.

SIN3A antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 μg) probed with SIN3A antibody at a dilution of 1:5,000.

for SIN3A antibody (pAb)Background

SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.

for SIN3A antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SIN3A antibody.

for SIN3A antibody (pAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Negative Control Primer Set 1 (Cat. No. 71011) has been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SIN3A antibody (pAb).

for SIN3A antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SIN3A antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SIN3A antibody (pAb)
 

Data Thumbnails

SIN3A antibody (pAb) tested by ChIP.

ChIP of SIN3A pAb.
(Click image to enlarge and see details.)

SIN3A antibody (pAb) tested by Western blot.

Western blot of SIN3A pAb.
(Click image to enlarge and see details.)