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SIN3A antibody (pAb)
| Catalog No: 39865 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39866 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:1,000 - 1:5,000 | — |
for SIN3A antibody (pAb)Background
SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.
for SIN3A antibody (pAb)Immunogen
This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A.
for SIN3A antibody (pAb)Application Notes
This SIN3A antibody has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution) and chromatin IP (5- 10 µl per ChIP).
for SIN3A antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for SIN3A antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SIN3A antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with SIN3A antibody (pAb).
SIN3A antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 μg) probed with SIN3A antibody at a dilution of 1:5,000.
SIN3A antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using Active Motif's Epigenetic Services protocol with 30 µg of chromatin from TM4 Sertoli cells and 5 µl of SIN3A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
for SIN3A antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for SIN3A antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
