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MOZ antibody (pAb)

Aliases: KAT6A, MYST3, ZNF220, RUNXBP2, ZC2HC6A
商品コード: 39867 Format: 100 µl ¥67,000 Buy Now
商品コード: 39868 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:290 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated

for MOZ antibody (pAb)Application Notes


Validated Applications:
WB: 1:200 - 1:500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Published Applications:
ChIP
WB

for MOZ antibody (pAb)Immunogen

This MOZ antibody was raised against a synthetic eptide within the N-terminal half of human MOZ (Monocytic leukemia zinc finger protein aka histone acetyltransferase MYST3).

for MOZ antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MOZ antibody (pAb)References

 
MOZ antibody (pAb) tested by Western blot.

MOZ antibody (pAb) tested by Western blot.
Nuclear extract of K-562 cells (40 μg) probed with MOZ antibody at a dilution of 1:500.

for MOZ antibody (pAb)Background

MOZ (Monocytic leukemia zinc finger protein) / MYST3 (also known KAT6A) is a histone acetyltransferase specific for H3. It is part of the MOZ/MORF protein complex and is involved in the activation of transcription. Specifically, it is known to interact with and serve as a co-activator for RUNX1 and RUNX2. MOZ / MYST3 is found concentrated in sub-nuclear foci that are distinct from PML bodies. Chromosomal aberrations involving MYST3 may be a cause of acute myeloid leukemias. MYST3-NCoA2 has been shown to bind CBP and disrupt its transcriptional activation function.

for MOZ antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for MOZ antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MOZ antibody (pAb).

for MOZ antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MOZ antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MOZ antibody (pAb)
 

Data Thumbnails

MOZ antibody (pAb) tested by Western blot.

Western blot of MOZ pAb.
(Click image to enlarge and see details.)