Ubn1 antibody (pAb)

for Ubn1 antibody (pAb)Background

UBN1 (Ubinuclein 1, also known as HIRA binding protein) is a ubiquitously expressed nuclear protein that acts as a novel regulator of senescence. UBN1 is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. It is expressed in a number of cancer cell lines and tumors and may be required for replication-independent chromatin assembly. UBN1 is part of a large multi-subunit protein complex that interacts with CEBPA and Epstein-Barr virus BLZF1. It co-localizes with HIRA, PML and SP100 in the PML bodies of senescent cells. When overexpressed, UBN1 accumulates in the nucleus of cells defective for cytokinesis.

for Ubn1 antibody (pAb)Immunogen

This Ubinuclein antibody was raised against a recombinant protein corresponding to amino acids 1-345 of human Ubn1.

for Ubn1 antibody (pAb)Application Notes

This Ubn1 antibody has been validated for use in Western blotting (1:500 - 1:1,000).

Addition of 0.05% Tween-20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for Ubn1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Ubn1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Ubn1 antibody.

for Ubn1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ubn1 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.