Histone macroH2A1.1 antibody (pAb)

for Histone macroH2A1.1 antibody (pAb)Background

Histone macroH2A1.1 is a histone H2A variant and one of the three members of the macroH2A family. The three macroH2A proteins contain the C-terminal 'macro' domain in addition to the H2A homologous region. This macro domain may bind poly ADP-ribose and other NAD+ metabolites. The macroH2A1.1 and macroH2A1.2 proteins are produced from alternatively spliced transcripts from the macroH2A1 gene while a second distinct macroH2A gene encodes macroH2A2. In mammals, macroH2A is preferentially localized to the inactive X chromosome and in general is proposed to play a role in repressing gene expression. In mice, macroH2A1 is required for the silencing of endogenous retroviruses.

for Histone macroH2A1.1 antibody (pAb)Immunogen

This macroH2A1.1 antibody was raised against a peptide corresponding to amino acids 198-226 of human macroH2A1.1.

for Histone macroH2A1.1 antibody (pAb)Application Notes

This Histone macrH2A1.1 antibody has been validated for use in Western blotting (1:500 - 1:1,000), immunohistochemistry and immunofluorescence. See references for more details.

The apparent molecular weight of the protein in the Western blot data below is larger than expected due to the inclusion of a GST epitope tag.

for Histone macroH2A1.1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Histone macroH2A1.1 antibody (pAb)References

This antibody has been used in the following references:
IF: Sporn J. et al (2009) Oncogene 8: 3423-3428.
IHC: Sporn J. et al (2009) Oncogene 8: 3423-3428.

for Histone macroH2A1.1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone macroH2A1.1 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.