Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

Histone macroH2A2 antibody (pAb)

商品コード: 39873 Format: 100 µg ¥67,000 Buy Now
商品コード: 39874 Format: 10 µg ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:40 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for Histone macroH2A2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications:
IHC
ICC/IF
See references for more information. Individual optimization may be required.

for Histone macroH2A2 antibody (pAb)Immunogen

This macroH2A2 antibody was raised against a peptide corresponding to amino acids 162-372 of human macroH2A2.

for Histone macroH2A2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone macroH2A2 antibody (pAb)References

 
Histone macroH2A2 antibody (pAb) tested by Western blot.

Histone macroH2A2 antibody (pAb) tested by Western blot.
U2OS cells mock transfected (lane 1) or transfected with GST-Tagged macroH2A1.1 (lane 2), GST-macroH2A1.2 (lane 3) or GST-macroH2A2 (lane 4) were probed with Histone macroH2A2 antibody at a dilution of 1:1,000.

for Histone macroH2A2 antibody (pAb)Background

Histone macroH2A2 is a histone H2A variant and one of the three members of the macroH2A family. The three macroH2A proteins contain the C-terminal 'macro' domain in addition to the H2A homologous region. This macro domain may bind poly ADP-ribose and other NAD+ metabolites. The macroH2A1.1 and macroH2A1.2 proteins are produced from alternatively spliced transcripts from the macroH2A1 gene while a second distinct macroH2A gene encodes macroH2A2. In mammals, macroH2A is preferentially localized to the inactive X chromosome and in general is proposed to play a role in repressing gene expression.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone macroH2A2 antibody (pAb).

for Histone macroH2A2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone macroH2A2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone macroH2A2 antibody (pAb)
 

Data Thumbnails

Histone macroH2A2 antibody (pAb) tested by Western blot.

Western blot of Histone macroH2A2 pAb.
(Click image to enlarge and see details.)