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HDAC1 antibody (mAb)

Clone: 10E2
Aliases: HD1, RPD3, GON-10, RPD3L1
商品コード: 39881 Format: 100 µg ¥67,000 Buy Now
商品コード: 39882 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:60 kDa
Reactivity:Human, Mouse, Rat

Applications

Western Blot Validated

for HDAC1 antibody (mAb) (Clone 10E2)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

for HDAC1 antibody (mAb) (Clone 10E2)Immunogen

This HDAC1 antibody was raised against a peptide corresponding to amino acids 467-482 of murine HDAC1.

for HDAC1 antibody (mAb) (Clone 10E2)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a concentrated supernatant version (Catalog No. 39531) of this antibody is also available.

for HDAC1 antibody (mAb) (Clone 10E2)References

 
HDAC1 antibody (mAb) tested by Western blot.

HDAC1 antibody (mAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with HDAC1 antibody at a dilution of 1 µg/ml.

for HDAC1 antibody (mAb) (Clone 10E2)Background

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for HDAC1 antibody (mAb) (Clone 10E2)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC1 antibody.

for HDAC1 antibody (mAb) (Clone 10E2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HDAC1 antibody (mAb) (Clone 10E2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HDAC1 antibody (mAb)
 

Data Thumbnails

HDAC1 antibody (mAb) tested by Western blot.

Western blot of HDAC1 mAb.
(Click image to enlarge and see details.)