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HDAC2 antibody (mAb)

Clone: 3F3
Aliases: HD2, RPD3, YAF1
商品コード: 39883 Format: 100 µg ¥67,000 Buy Now
商品コード: 39884 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:55 kDa
Reactivity:Human, Mouse, Rat

Applications

Western Blot Validated

for HDAC2 antibody (mAb) (Clone 3F3)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

for HDAC2 antibody (mAb) (Clone 3F3)Immunogen

This HDAC2 antibody was raised against a peptide corresponding to amino acids 473-488 of human HDAC2.

for HDAC2 antibody (mAb) (Clone 3F3)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a concentrated supernatant version (Catalog No. 39533) of this antibody is also available.

for HDAC2 antibody (mAb) (Clone 3F3)References

 
HDAC2 antibody (mAb) tested by Western blot.

HDAC2 antibody (mAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with HDAC2 antibody at a dilution of 1 µg/ml.

for HDAC2 antibody (mAb) (Clone 3F3)Background

HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for HDAC2 antibody (mAb) (Clone 3F3)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC2 antibody.

for HDAC2 antibody (mAb) (Clone 3F3)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HDAC2 antibody (mAb) (Clone 3F3)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HDAC2 antibody (mAb)
 

Data Thumbnails

HDAC2 antibody (mAb) tested by Western blot.

Western blot of HDAC2 mAb.
(Click image to enlarge and see details.)