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GATA-4 antibody (pAb)

Aliases: ASD2, VSD1, TACHD
商品コード: 39893 Format: 100 µl ¥67,000 Buy Now
商品コード: 39894 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:55 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for GATA-4 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for GATA-4 antibody (pAb)Immunogen

This GATA-4 antibody was raised against a peptide derived from the C-terminus of human GATA-4.

for GATA-4 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for GATA-4 antibody (pAb)References

 
GATA-4 antibody (pAb) tested by Western blot.

GATA-4 antibody (pAb) tested by Western blot.
Western Blot: Nuclear extract (20 µg) of F9 cells (retinoic acid treated mouse teratocarcinoma F9 stem cell line, Active Motif Catalog no. 36113) probed with GATA-4 antibody at a dilution of 1:2,000.

for GATA-4 antibody (pAb)Background

GATA-4 is a zinc finger protein and part of the GATA family of sequence specific DNA binding transcription factors that recognize a consensus DNA sequence containing GATA. GATA-4 is involved in the development of several mesodermal and endodermal structures. GATA-4 mutant mice exhibit severe heart malformations. Defects in GATA-4 are the cause of atrial septal defect type 2. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. GATA-4 also plays a role in regulating gene expression in cardiac and smooth muscle cell types.

for GATA-4 antibody (pAb)Positive Control

Active Motif's F9 nuclear extract (Retinoic acid treated) (Catalog No. 36113) can be used as a positive control for GATA-4 antibody.

for GATA-4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for GATA-4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

GATA-4 antibody (pAb)
 

Data Thumbnails

GATA-4 antibody (pAb) tested by Western blot.

Western blot of GATA-4 pAb.
(Click image to enlarge and see details.)