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DNMT1 antibody (pAb)

Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
商品コード: 39905 Format: 100 µg ¥67,000 Buy Now
商品コード: 39906 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:185 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for DNMT1 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µg per IP
WB: 0.5 - 2 µg/ml dilution

Published Applications:
WB

for DNMT1 antibody (pAb)Immunogen

This DNMT1 antibody was raised against a recombinant protein corresponding to amino acids 1-110 of human DNMT1.

for DNMT1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DNMT1 antibody (pAb)References

 
DNMT1 antibody (pAb) tested by immunoprecipitation.

DNMT1 antibody (pAb) tested by Immunoprecipitation.
10 µg of DNMT1 antibody was used to immunoprecipitate DNMT1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DNMT1 antibody at a dilution of 0.5 µg/ml.

DNMT1 antibody (pAb) tested by Western blot.

DNMT1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with DNMT1 antibody (pAb) at a dilution of 0.5 µg/ml.

for DNMT1 antibody (pAb)Background

DNMT1DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

for DNMT1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for DNMT1 antibody.

for DNMT1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DNMT1 antibody (pAb)
 

Data Thumbnails

DNMT1 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of DNMT1 pAb.
(Click image to enlarge and see details.)

DNMT1 antibody (pAb) tested by Western blot.

Western blot of DNMT1 pAb.
(Click image to enlarge and see details.)