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MAZ antibody (mAb)

Clone: 133
Aliases: PUR1, ZF87, Pur-1, SAF-1, SAF-2, SAF-3, Zif87, ZNF801
商品コード: 39935 Format: 100 µg ¥67,000 Buy Now
商品コード: 39936 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:55 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Electrophoretic Mobility Shift Assay Validated

for MAZ antibody (mAb) (Clone 133)Application Notes


Validated Applications:
WB

Published Applications:
ChIP
EMSA
See references for more information. Individual optimization may be required.

for MAZ antibody (mAb) (Clone 133)Immunogen

This MAZ antibody was raised against recombinant human MAZ protein.

for MAZ antibody (mAb) (Clone 133)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MAZ antibody (mAb) (Clone 133)References

 
MAZ antibody (mAb) tested by Western Blot.

MAZ antibody (mAb) tested by Western Blot.
10 µg MCF7 cell nuclear extract was run on SDS-PAGE, and probed with 2 µg/ml AbFlex MAZ antibody.
MW: 55 kDa

for MAZ antibody (mAb) (Clone 133)Background

MAZ (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ antibody (mAb) tested by Western Blot.
10 µg MCF7 cell nuclear extract was run on SDS-PAGE, and probed with 2 µg/ml AbFlex MAZ antibody.
MW: 55 kDa
binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. MAZ also participates in skeletal muscle development through the activation of several skeletal muscle-specific genes. MAZ has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MAZ antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MAZ antibody (mAb).

for MAZ antibody (mAb) (Clone 133)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MAZ antibody (mAb) (Clone 133)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MAZ antibody (mAb)
 

Data Thumbnails

MAZ antibody (mAb) tested by Western Blot.

Western Blot testing of MAZ antibody (mAb).
(Click to enlarge and view details).