Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

SREBP-1 antibody (mAb)

Clone: IgG-2A4
Aliases: Sterol regulatory element-binding protein 1, SREBF1, bHLHd1, SREBP-1c
商品コード: 39939 Format: 100 µg ¥67,000 Buy Now
商品コード: 39940 Format: 10 µg ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:68 and 125 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Electrophoretic Mobility Shift Assay Validated

for SREBP-1 antibody (mAb) (Clone IgG-2A4)Application Notes


Published Applications:
IP
EMSA
WB
See references for more information. Individual optimization may be required.

for SREBP-1 antibody (mAb) (Clone IgG-2A4)Immunogen

This SREBP-1 antibody was raised against a recombinant protein corresponding to amino acids 301-407 of human SREBP-1.

for SREBP-1 antibody (mAb) (Clone IgG-2A4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SREBP-1 antibody (mAb) (Clone IgG-2A4)References

for SREBP-1 antibody (mAb) (Clone IgG-2A4)Background

SREBP-1 and SREBP-2 (Sterol regulatory element-binding protein 1 and 2) are members of the basic-helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors and are involved in the regulation of cholesterol homeostasis. SREBP-1 and SREBP-2 are normally membrane bound at the ER and nuclear envelope, but in sterol-depleted cells, the membrane-bound precursors are cleaved to generate a soluble isoforms that translocate to the nucleus. These active forms, which include the bHLH-ZIP domain, activate transcription of genes involved in the uptake and synthesis of cholesterol.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use SREBP-1 antibody (mAb).

for SREBP-1 antibody (mAb) (Clone IgG-2A4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SREBP-1 antibody (mAb) (Clone IgG-2A4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SREBP-1 antibody (mAb)