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SREBP-2 antibody (mAb)

Clone: IgG-1C6
Aliases: Sterol regulatory element-binding protein 2, SREBF2, bHLHd2, SREBP-2
商品コード: 39941 Format: 100 µg ¥67,000 Buy Now
商品コード: 39942 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:68 and 125 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Application Notes


Published Applications:
ChIP
WB
See references for more information. Individual optimization may be required.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Immunogen

This SREBP-2 antibody was raised against a recombinant protein corresponding to amino acids 833-1141 of human SREBP-2.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)References

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Background

SREBP-1 and SREBP-2 (Sterol regulatory element-binding protein 1 and 2) are members of the basic-helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors and are involved in the regulation of cholesterol homeostasis. SREBP-1 and SREBP-2 are normally membrane bound at the ER and nuclear envelope, but in sterol-depleted cells, the membrane-bound precursors are cleaved to generate a soluble isoforms that translocate to the nucleus. These active forms, which include the bHLH-ZIP domain, activate transcription of genes involved in the uptake and synthesis of cholesterol.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SREBP-2 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use SREBP-2 antibody (mAb).

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SREBP-2 antibody (mAb) (Clone IgG-1C6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SREBP-2 antibody (mAb)