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Histone H4ac (pan-acetyl) antibody (mAb)

Clone: 3HH4-2C2
商品コード: 39967 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)Application Notes


Published Applications:
ICC/IF
WB

See references for more information. Individual optimization may be required.

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)Immunogen

This Histone H4 acetyl antibody was raised against a peptide corresponding to amino acids 1- 24 of human histone H4 acetylated at lysines 5, 8, 12 and 16. Due to sequence similarity, cross-reactivity has been observed with acetylated Histone H2B.

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)References

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 pan-acetyl antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4ac (pan-acetyl) antibody (mAb).

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 pan-acetyl antibody (mAb) (Clone 3HH4-2C2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4ac (pan-acetyl) antibody (mAb)