RAR-γ antibody (mAb)

for RAR-gamma antibody (mAb) (Clone 4g-7A11)Background

Retinoic acid receptors (RAR) are nuclear receptors that are activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-α, RAR-β and RAR-γ. RAR-γ has two splice isoforms. RAR-γ heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements complexed with co-repressor protein. Binding of agonist ligands to RAR results in dissociation of co-repressor and recruitment of coactivator protein which in turn promotes transcription of the downstream target gene into mRNA and eventually protein.

for RAR-gamma antibody (mAb) (Clone 4g-7A11)Immunogen

This RAR-γ antibody was raised against a recombinant protein corresponding to amino acids 428-441 (F-region) of human RARγ.

for RAR-gamma antibody (mAb) (Clone 4g-7A11)Application Notes

This RAR-γ antibody has been validated for use in ELISA, EMSA (2 - 10 μg per EMSA), immunocytochemistry, immunofluorescence (2 - 5 μg/ml), immunohistochemistry (0.5 - 2 μg/ml), immunoprecipitation (2 - 10 μg per IP) and Western blotting (0.5 - 2 μg/ml). See references for details.

for RAR-gamma antibody (mAb) (Clone 4g-7A11)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for RAR-gamma antibody (mAb) (Clone 4g-7A11)References

This antibody has been used in the following references:
IHC: Reichrath J et al (1997) Histochem J 29: 127-133; Hoftijzer HC et al (2009) Eur J Endocrinol 160: 631-638.

for RAR-gamma antibody (mAb) (Clone 4g-7A11)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for RAR-gamma antibody (mAb) (Clone 4g-7A11)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.