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Antibodies

HP1γ antibody (mAb)

Clone: 2MOD-1G6
Catalog No: 39981 Format: 100 µg $340 Buy Now
Catalog No: 39982 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1k
Purification:Protein G Chromatography
Applications:ELISA, ICC, IF, IHC, IP, WB
Reactivity:Human, Mouse
Molecular Weight:21 kDa

Applications Table

Western Blot Chromatin IP IF IP
0.5 - 2 µg/ml — 0.5 - 2 µg/ml 2 - 10 µg per IP

for HP1 gamma antibody (mAb) (Clone 2MOD-1G6)Background

Heterochromatin Protein 1 gamma (HP1 gamma) is one of the mammalian homologues of Drosophila HP1, first identified through its role in the maintenance of regions of the genome in a heterochromatic state. HP1 gamma contains a chromo domain and a chromo shadow domain. Through the chromo domain, HP1 proteins are recruited to regions of chromatin that exhibit methylation of lysine 9 of histone H3.

for HP1 gamma antibody (mAb) (Clone 2MOD-1G6)Immunogen

This HP1γ antibody was raised against recombinant full-length mouse HP1γ.

for HP1 gamma antibody (mAb) (Clone 2MOD-1G6)Application Notes

This HP1 gamma antibody has been validated for use in ELISA, immunocytochemistry (0.5 - 2 µg/ml), immunofluorescence (0.5 - 2 µg/ml), immunohistochemistry (0.5 - 2 µg/ml), immunoprecipitation (2 - 10 µg per IP) and Western blotting (0.5 - 2 µg/ml). See references for details.

for HP1 gamma antibody (mAb) (Clone 2MOD-1G6)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use HP1γ antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HP1γ antibody (mAb).

for HP1 gamma antibody (mAb) (Clone 2MOD-1G6)References

IF: Nielsen AL et al (1999) EMBO J 22: 6385-6395.
WB: Nielsen AL et al (1999) EMBO J 22: 6385-6395.

for HP1 gamma antibody (mAb) (Clone 2MOD-1G6)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HP1 gamma antibody (mAb) (Clone 2MOD-1G6)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

HP1γ antibody (mAb)