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DAX-1 / NR0B1 antibody (mAb)

Clone: 1DA-2F4
Aliases: AHC, AHX, DSS, GTD, HHG, AHCH, DAX1, NROB1, SRXY2
商品コード: 39983 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:52 kDa
Reactivity:Human, Mouse, Rat

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Application Notes


Published Applications:
ICC/IF
IHC
WB
See references for more information. Individual optimization may be required.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Immunogen

This DAX-1 / NR0B1 antibody was raised against a peptide corresponding to amino acids 135-166 of human DAX-1 / NR0B1.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)References

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Background

DAX-1 (DSS-AHC critical region on the X chromosome protein 1, NR0B1, Nuclear receptor subfamily 0 group B member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. DAX-1 / NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. It may also have a role in embryonic development and in the maintenance of embryonic stem cell pluripotency as it up-regulates Oct-4 expression ES cells. Defects in DAX-1 are a cause of dosage-sensitive sex reversal (DSSR) and X-linked adrenal hypoplasia congenital (XL-AHC). DSSR results from a duplication of the region of the X-chromosome containing DAX-1, resulting in males with two copies of DAX-1, which inhibits normal testis formation, and thus XY individuals develop as females. XL-AHC is a developmental disorder of the adrenal gland, characterized by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with DAX-1 / NR0B1 antibody (mAb).

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DAX-1 / NR0B1 antibody (mAb)