Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

MyoD antibody (mAb)

Clone: 5F11
Catalog No: 39991 Format: 100 µg $340 Buy Now
Catalog No: 39992 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Rat
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:IF, WB
Reactivity:Human, Mouse
Molecular Weight:40 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
Published.
See Application Notes below.
Published.
See Application Notes below.

for MyoD antibody (mAb) (Clone 5F11)Background

MyoD (Myogenic differentiation factor 1, Myf-3), is a transcription factor belonging to the myogenic factors family that contains a basic helix-loop-helix (bHLH) DNA-binding domain. MyoD is the central regulator in the signaling pathways that regulate muscle development.

for MyoD antibody (mAb) (Clone 5F11)Immunogen

This MyoD antibody was raised against a recombinant protein corresponding to full length mouse MyoD.

for MyoD antibody (mAb) (Clone 5F11)Application Notes

This MyoD antibody has been validated for use in immunofluorescence and Western blot. See references for details.

for MyoD antibody (mAb) (Clone 5F11)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MyoD antibody (mAb).

for MyoD antibody (mAb) (Clone 5F11)References

This antibody has been used in the following references:
IF: Harada A et al (2010) Hybridoma 29: 255-258.
WB: Harada A et al (2010) Hybridoma 29: 255-258

for MyoD antibody (mAb) (Clone 5F11)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MyoD antibody (mAb) (Clone 5F11)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

MyoD antibody (mAb)