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Antibodies

CAF-1 p150 antibody (mAb)

Clone: 1
Catalog No: 39997 Format: 100 µg $340 Buy Now
Catalog No: 39998 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Hamster
Isotype:IgG1
Purification:Protein G Chromatography
Applications:IF, IP, WB
Reactivity:Human
Molecular Weight:150 kDa

Applications Table

Western Blot Chromatin IP IF IP
0.5 - 2 µg/ml — 0.5 - 2 µg/ml 2 - 10 µg per IP

for CAF-1 p150 antibody (mAb) (Clone 1)Background

CAF-1 p150 (Chromatin assembly factor-1 p150 subunit, CAF1A, CHAF1A) is the largest subunit of the CAF-1 complex that is required for the assembly of nucleosomes onto newly replicated DNA. Specifically, CAF-1 assists in the loading of the H3-H4 tetramer and may be involved in DNA damage repair.

for CAF-1 p150 antibody (mAb) (Clone 1)Immunogen

The CAF-1 p150 antobody was raised against full length CAF-1 purified from human 293 cells.

for CAF-1 p150 antibody (mAb) (Clone 1)Application Notes

This CAF-1 p150 antibody has been validated for use in immunofluorescence (0.5 - 2 µg/ml), immunoprecipitation (2 - 10 µg per IP) and Western blotting (0.5 - 2 µg/ml). See references for details.

for CAF-1 p150 antibody (mAb) (Clone 1)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CAF-1 p150 antibody (mAb).

for CAF-1 p150 antibody (mAb) (Clone 1)References

This antibody has been used in the following references:
IF: Shibahara K and Stillman B (1999) Cell 96: 575-585; Smith S and Stillman B (1991) J Biol Chem 266: 12041-12047; Hoek M and Stillman B (2003) PNAS 266: 12183-12188.
IP: Kaufman PD et al (1995) Cell 81: 1105-1114.
WB: Kaufman PD et al (1995) Cell 81: 1105-1114.

for CAF-1 p150 antibody (mAb) (Clone 1)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for CAF-1 p150 antibody (mAb) (Clone 1)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

CAF-1 p150 antibody (mAb)