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IκBα antibody (mAb)

Clone: 6A920
Aliases: NFKBIA, MAD-3, NFKBI
商品コード: 40903 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:40 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for IkBa antibody (mAb) (Clone 6A920)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications:
WB

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IkBa antibody (mAb) (Clone 6A920)Immunogen

This IκBα antibody was raised against a recombinant protein corresponding to amino acid residues 32-291 of human IκBα.

for IkBa antibody (mAb) (Clone 6A920)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IkBa antibody (mAb) (Clone 6A920)References

 
IκBα antibody (mAb) tested by Western blot.

IκBα mAb tested by Western blot.
The anti-IκBα detects both phosphorylated and non-phosphorylated forms of IκBα by Western blot. Jurkat cells (~1 x 107) were treated for indicated time periods with 10 nm/ml PMA, 1 µM ionomycin and anti-CD28 (1:10,000 dilution). 10 µl of cytoplasmic (C) and nuclear extract (N) were resolved on a 8.75% SDS-PAGE and transferred to Immobilon membrane. Figure is courtesy of Dr. Shao-Cong Sun at Penn State Univ. College of Medicine.

for IkBa antibody (mAb) (Clone 6A920)Background

IκBα – NFκB (NFκB p50 and NFκB p65) signaling is controlled to a large extent by the sequestration of the NFκB complex in the cytoplasm by its association with one of the IκB family of proteins. IκBα is phosphorylated at Ser32 and Ser36 (IκBα phospho Ser32,36) by the IκB Kinase (IKK) complex, resulting in the degradation of IκB and the nuclear translocation of NFκB.

for IkBa antibody (mAb) (Clone 6A920)Positive Control

Active Motif's Jurkat nuclear extract (TPA + CI stimulated) (Catalog No. 36013) can be used as a positive control for IκBα antibody.

for IkBa antibody (mAb) (Clone 6A920)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IkBa antibody (mAb) (Clone 6A920)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

IκBα antibody (mAb)
 

Data Thumbnails

IκBα antibody (mAb) tested by Western blot.

Western blot of IκBα mAb.
(Click image to enlarge and see details.)