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IKKα antibody (mAb)

Clone: 14A231
Aliases: CHUK, IKK1, IKBKA, TCF16, NFKBIKA, IKK-alpha
商品コード: 40905 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:85 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for IKKa antibody (mAb) (Clone 14A231)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IKKa antibody (mAb) (Clone 14A231)Immunogen

This IKKα antibody was raised against a full-length protein corresponding to human IKKα.

for IKKa antibody (mAb) (Clone 14A231)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKa antibody (mAb) (Clone 14A231)References

 
IKKα antibody (mAb) tested by Western blot.

IKKα mAb tested by Western blot.
IKKα detection by Western blot analysis. The detection of IKKα was performed using 30 µg of whole-cell extract from Daudi cells with the IKKα mAb at a 1 µg/ml dilution.

for IKKa antibody (mAb) (Clone 14A231)Background

IKKα (IκB Kinase alpha subunit) – The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB (NFκB p50 and NFκB p65). The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ).

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IκBα at Ser32 and Ser36. This results ultimately in the degradation of IκB and the activation and nuclear translocation of NFκB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK-beta appears to be the principal kinase, as IKKα is not required for activation of the IKK complex and the subsequent degradation of IκBα.

for IKKa antibody (mAb) (Clone 14A231)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IKKa antibody (mAb) (Clone 14A231)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

IKKα antibody (mAb)
 

Data Thumbnails

IKKα antibody (mAb) tested by Western blot.

Western blot of IKKα mAb.
(Click image to enlarge and see details.)