IKKβ antibody (mAb)

for IKKb antibody (mAb) (Clone 10AG2)Background

IKKβ (IκB Kinase beta subunit) – The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB (NFκB p50 and NFκB p65). The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ).

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IκBα at Ser32 and Ser36. This results ultimately in the degradation of IκB and the activation and nuclear translocation of NFκB.

While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKα is not required for activation of the IKK complex and the subsequent degradation of IκBα. Once activated, IKKβ autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.

for IKKb antibody (mAb) (Clone 10AG2)Immunogen

This antibody was raised against a 6xHis tag full-length protein corresponding to human IKKβ.

for IKKb antibody (mAb) (Clone 10AG2)Application Notes

This IKKβ antibody (Clone 10AG2) has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IKKb antibody (mAb) (Clone 10AG2)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKb antibody (mAb) (Clone 10AG2)Positive Control

Active Motif's Daudi nuclear extract (Catalog No. 36091) can be used as a positive control for IKKβ antibody (Clone 10AG2).

for IKKb antibody (mAb) (Clone 10AG2)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for IKKb antibody (mAb) (Clone 10AG2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.