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IKKβ antibody (mAb)

Clone: 10A9B6
Aliases: IKBKB, IKK2, IMD15, NFKBIKB, IKK-beta
商品コード: 40907 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:87 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for IKKb antibody (mAb) (Clone 10A9B6)Application Notes


Validated Applications:
IP: 2 µg per IP
WB: 1 - 2 µg/ml dilution

Individual optimization may be required.

Published Applications:
WB

for IKKb antibody (mAb) (Clone 10A9B6)Immunogen

This IKKβ antibody was raised against a 6xHis-Tag full-length protein corresponding to human IKKβ.

for IKKb antibody (mAb) (Clone 10A9B6)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKb antibody (mAb) (Clone 10A9B6)References

 
IKKβ antibody (mAb) tested by immunoprecipitation.

IKKβ mAb (Clone 10A9B6) tested by Western blot.
Immunoprecipitation of IKKβ. 2 µg of IKKβ mAb (Clone 10A9B6) was used to immunoprecipitate IKKβ from 1 x 106 cells. The immunoprecipitated protein was detected by Western blotting using the same antibody. The IKKβ protein detected was ~87 kDa in size.

for IKKb antibody (mAb) (Clone 10A9B6)Background

IKKβ (IκB Kinase beta subunit) – The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB (NFκB p50 and NFκB p65). The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ).

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IκBα at Ser32 and Ser36. This results ultimately in the degradation of IκB and the activation and nuclear translocation of NFκB.

While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKα is not required for activation of the IKK complex and the subsequent degradation of IκBα. Once activated, IKKβ autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use IKKβ antibody (mAb).

for IKKb antibody (mAb) (Clone 10A9B6)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IKKb antibody (mAb) (Clone 10A9B6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

IKKβ antibody (mAb)
 

Data Thumbnails

IKKβ antibody (mAb) tested by immunoprecipitation.

Immunoprecipitation of IKKβ mAb.
(Click image to enlarge and see details.)