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IKKγ antibody (mAb)

Clone: 46B844
Aliases: IKBKG, IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, ZC2HC9, IKK-gamma
商品コード: 40908 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:48 kDa
Reactivity:Human

Applications

Western Blot Validated

for IKKg antibody (mAb) (Clone 46B844)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IKKg antibody (mAb) (Clone 46B844)Immunogen

This IKKγ antibody was raised against a full-length protein corresponding to human IKKγ.

for IKKg antibody (mAb) (Clone 46B844)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKg antibody (mAb) (Clone 46B844)References

 
IKKγ antibody (mAb) tested by Western blot.

IKKγ mAb tested by Western blot.
IKKγ detection by Western blot. IKKγ was detected in 30 µg of whole-cell extract from Daudi cells with IKKγ mAb at a 2 µg/ml dilution.

for IKKg antibody (mAb) (Clone 46B844)Background

IKKβ (IκB Kinase beta subunit) – The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB (NFκB p50 and NFκB p65). The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ).

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IκBα at Ser32 and Ser36. This results ultimately in the degradation of IκB and the activation and nuclear translocation of NFκB.

While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKα is not required for activation of the IKK complex and the subsequent degradation of IκBα. Once activated, IKKβ autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.

for IKKg antibody (mAb) (Clone 46B844)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IKKg antibody (mAb) (Clone 46B844)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

IKKγ antibody (mAb)
 

Data Thumbnails

IKKγ antibody (mAb) tested by Western blot.

Western blot of IKKγ mAb.
(Click image to enlarge and see details.)