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NFκB p65 antibody (mAb)

Clone: 112A1021
Aliases: RELA, p65, NFKB3
商品コード: 40916 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Affinity Purified
Host:Mouse
Molecular Weight:65 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated

for NFkB p65 antibody (mAb) (Clone 112A1021)Application Notes


Validated Applications:
WB: 2 - 5 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Published Applications:

ChIP

for NFkB p65 antibody (mAb) (Clone 112A1021)Immunogen

This NFkB p65 antibody was raised against a synthetic peptide corresponding to amino acid residues 526-539 of human NFκB p65.

for NFkB p65 antibody (mAb) (Clone 112A1021)Buffer

PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

for NFkB p65 antibody (mAb) (Clone 112A1021)References

 
NFκB p65 antibody (mAb) tested by Western blot.

NFκB p65 mAb tested by Western blot.
NFκB p65 detection by Western blot. The analysis was performed using Daudi whole-cell extract and NFκB p65 mAb at a 2 µg/ml dilution.

for NFkB p65 antibody (mAb) (Clone 112A1021)Background

NFκB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFκB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1)and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with NFκB p65 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use NFκB p65 antibody (mAb).

for NFkB p65 antibody (mAb) (Clone 112A1021)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NFkB p65 antibody (mAb) (Clone 112A1021)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NFκB p65 antibody (mAb)
 

Data Thumbnails

NFκB p65 antibody (mAb) tested by Western blot.

Western blot of NFκB p65 mAb.
(Click image to enlarge and see details.)