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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 2 - 4 µg/ml | — |
for Caspase-3 antibody (mAb)Background
Caspase-3 (CASP3 or apoptosis-related cysteine peptidase 3) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-3 cleaves and activates Caspase-6, Caspase-7 and Caspase-9, and the protein itself is processed by Caspase-8, Caspase-9 and Caspase-10. Caspase-3 is the predominate caspase involved in the cleavage of amyloid-beta 4A, which is associated with Alzheimer's disease.
for Caspase-3 antibody (mAb)Immunogen
This antibody was raised against a 6xHis-tagged, full-length human Caspase-3 protein.
for Caspase-3 antibody (mAb)Application Notes
This Caspase-3 antibody has been validated for use in Western blotting (2 - 4 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for Caspase-3 antibody (mAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for Caspase-3 antibody (mAb)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Caspase-3 antibody.
Caspase-3 mAb tested by Western blot.
Western blot analysis for detection of Caspase-3 activation. HeLa cells were treated with 2 µM staurosporine for different time periods and then probed using Caspase-3 mAb. Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively.
for Caspase-3 antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Caspase-3 antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
