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DRAK2 antibody (pAb)

Aliases: STK17B
商品コード: 40926 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:76 kDa
Reactivity:Human

Applications

Western Blot Validated

for DRAK2 antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for DRAK2 antibody (pAb)Immunogen

This DRAK2 antibody was raised against a peptide corresponding to amino acid residues 351-365 of human DRAK2.

for DRAK2 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for DRAK2 antibody (pAb)References

 
DRAK2 antibody (pAb) tested by Western blot.

DRAK2 pAb tested by Western blot.
Detection of DRAK2 by Western blot. The analysis was performed using 30 µg HeLa whole-cell extract and DRAK2 pAb at a 2 µg/ml dilution.

for DRAK2 antibody (pAb)Background

Overexpression of DRAK2 (STK17B or DAP kinase-related apoptosis-inducing protein kinase 2) and ZIP kinase can cause the morphological changes typical of apoptosis in NIH/3T3 cells. Both DRAKs and ZIP kinase share significant homology at the amino acid level. The kinase domains of both DRAKs and ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-γ. Overexpression of DRAKs in NIH/3T3 cells lead to apoptosis. These experiments also indicate that the C-terminal domain of DRAKs are important for induction of apoptosis.

for DRAK2 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for DRAK2 antibody.

for DRAK2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DRAK2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DRAK2 antibody (pAb)
 

Data Thumbnails

DRAK2 antibody (pAb) tested by Western blot.

Western blot of DRAK2 pAb.
(Click image to enlarge and see details.)