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Caspase-9 antibody (pAb) (Pro-form)

Aliases: CASP9
商品コード: 40931 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:46 kDa
Reactivity:Human

Applications

Western Blot Validated

for Caspase-9 antibody (pAb) (Pro-form)Application Notes


Validated Applications:
WB: 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Caspase-9 antibody (pAb) (Pro-form)Immunogen

This Caspase-9 antibody (Pro-form) was raised against a peptide corresponding to amino acid residues 299 -318 of human Pro-Caspase-9.

for Caspase-9 antibody (pAb) (Pro-form)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Caspase-9 antibody (pAb) (Pro-form)References

 
Caspase-9 antibody (pAb) (Pro-form) tested by Western blot.

Caspase-9 pAb (Pro-form) tested by Western blot.
Caspase-9 detection by Western blot. The analysis was performed using 50 µg HeLa whole-cell extract and Caspase-9 pAb (Pro-form) at a 1:1,000 dilution.

for Caspase-9 antibody (pAb) (Pro-form)Background

Caspase-9 (CASP9 or apoptosis-related cysteine peptidase 9) (Pro-form) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-9 is processed by caspase APAF1, which is thought to be one of the earliest events in the caspase activation cascade. Caspase-9 is then involved in processing of Caspase-3.

for Caspase-9 antibody (pAb) (Pro-form)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Caspase-9 antibody (Pro-form).

for Caspase-9 antibody (pAb) (Pro-form)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Caspase-9 antibody (pAb) (Pro-form)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Caspase-9 antibody (pAb) (Pro-form)
 

Data Thumbnails

Caspase-9 antibody (pAb) (Pro-form) tested by Western blot.

Western blot of Caspase-9 pAb (Pro-form).
(Click image to enlarge and see details.)