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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 2 - 4 µg/ml | — |
for DR4 antibody (mAb)Background
DR4 (also known as Tumor Necrosis Factor Receptor Superfamily, member 10a, TNFRSF10A, or TRAILR1) is a member of the TNF-receptor superfamily that is activated by TRAIL and induces apoptosis.
for DR4 antibody (mAb)Immunogen
This DR4 antibody was raised against a peptide corresponding to amino acid residues 1-20 of human DR4.
for DR4 antibody (mAb)Application Notes
This DR4 antibody has been validated for use in Western blotting (2 - 4 µg/ml dilution), and reported to work in flow cytometry. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for DR4 antibody (mAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
DR4 mAb tested by Western blot.
Detection of DR4 by Western blot. The analysis was performed using Daudi whole-cell extract and DR4 mAb at a 2 µg/ml dilution.
for DR4 antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for DR4 antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
