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TLR8 antibody (mAb)

Clone: 44C143
Aliases: CD288
商品コード: 40955 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:120 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for TLR8 antibody (mAb) (Clone 44C143)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for TLR8 antibody (mAb) (Clone 44C143)Immunogen

This TLR8 antibody was raised against a peptide corresponding to amino acid residues 750-850 of human TLR8.

for TLR8 antibody (mAb) (Clone 44C143)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for TLR8 antibody (mAb) (Clone 44C143)References

 
TLR8 antibody (mAb) tested by Western blot.

TLR8 mAb tested by Western blot.
TLR8 detection by Western blot. The analysis was performed using TLR8 mAb and nuclear extract from 293 cells that had been transfected with human TLR8 cDNA.

for TLR8 antibody (mAb) (Clone 44C143)Background

TLR8Toll-Like receptor 8 is a member of the Toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize specific molecular features associated with pathogens, and mediate the production of cytokines necessary for the development of effective immunity. Many of the TLRs heterodimerize with each other to modulate receptor function. TLR8 expression cells is elevated after exposure to both Gram-positive and Gram-negative bacteria. While primarily extracellular, TLR8 may be also localized intracellularly.

for TLR8 antibody (mAb) (Clone 44C143)Positive Control

Extracts from 293 cells transfected with human TLR8 cDNA can be used as a positive control for TLR8 antibody.

for TLR8 antibody (mAb) (Clone 44C143)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TLR8 antibody (mAb) (Clone 44C143)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TLR8 antibody (mAb)
 

Data Thumbnails

TLR8 antibody (mAb) tested by Western blot.

Western blot of TLR8 mAb.
(Click image to enlarge and see details.)