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Livin/ML-IAP antibody (mAb)

Clone: 88C570
Aliases: BIRC7, KIAP, MLIAP, RNF50
商品コード: 40958 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:31 kDa
Reactivity:Human

Applications

Western Blot Validated Immunohistochemistry Validated

for Livin/ML-IAP antibody (mAb) (Clone 88C570)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications:
WB
IHC

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Livin/ML-IAP antibody (mAb) (Clone 88C570)Immunogen

This Livin/ML-IAP antibody was raised against a GST-fusion protein corresponding to human Livin/ML-IAP.

for Livin/ML-IAP antibody (mAb) (Clone 88C570)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Livin/ML-IAP antibody (mAb) (Clone 88C570)References

 
Livin/ML-IAP antibody (mAb) tested by Western blot.

Livin/ML-IAP mAb tested by Western blot.
Livin detection by Western blot. The detection of Livin was performed using extract from a cell that had been transfected with human Livin cDNA. A protein of 31 kDa was detected using Livin/ML-IAP mAb at a 2 µg/ml dilution.

for Livin/ML-IAP antibody (mAb) (Clone 88C570)Background

Livin (ML-IAP or BIRC7) protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, Livin (ML-IAP or BIRC7) inhibits Caspase-3 and proteolytic activation of pro-Caspase-9. Isoform 1 of Livin (ML-IAP or BIRC7) blocks staurosporine-induced apoptosis, while isoform 2 blocks etoposide-induced apoptosis.

for Livin/ML-IAP antibody (mAb) (Clone 88C570)Positive Control

Extracts from cells transfected with human Livin cDNA can be used as a positive control for Livin/ML-IAP antibody.

for Livin/ML-IAP antibody (mAb) (Clone 88C570)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Livin/ML-IAP antibody (mAb) (Clone 88C570)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Livin/ML-IAP antibody (mAb)
 

Data Thumbnails

Livin/ML-IAP antibody (mAb) tested by Western blot.

Western blot of Livin/ML-IAP mAb.
(Click image to enlarge and see details.)