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TRAIL antibody (mAb)

Clone: 55B709.3
Aliases: TNFSF10, TL2, APO2L, CD253, Apo-2L
商品コード: 40966 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:32 kDa
Reactivity:Human

Applications

Western Blot Validated

for TRAIL antibody (mAb) (Clone 55B709.3)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for TRAIL antibody (mAb) (Clone 55B709.3)Immunogen

This TRAIL antibody was raised against a peptide corresponding to amino acid residues 17-35 of human TRAIL.

for TRAIL antibody (mAb) (Clone 55B709.3)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for TRAIL antibody (mAb) (Clone 55B709.3)References

 
TRAIL antibody (mAb) tested by Western blot.

TRAIL mAb tested by Western blot.
TRAIL detection by Western blot. The analysis was performed using 10 µg Jurkat whole-cell extract and TRAIL mAb at a 2 µg/ml dilution.

for TRAIL antibody (mAb) (Clone 55B709.3)Background

TRAIL (TNFSF10, CD253, Apo2L) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL is an acronym for TNF-Related Apoptosis-Inducing Ligand. Both the transmembrane and soluble recombinant forms of TRAIL can rapidly induce apoptosis in a wide variety of transformed and tumor cell lines, yet no cytotoxicity is observed with normal human cell lines, even though TRAIL and its receptors are expressed at significant levels there, and in most normal tissues in vitro. TRAIL is expressed constitutively in many tissues such as liver, lung, placenta, kidney, spleen, peripheral lymphocyte, etc. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, Caspase-3, and Caspase-8. TRAIL induces cancer apoptosis independently of p53 gene mutations. Two additional TRAIL decoy receptors (DcR1 and DcR2) do not trigger an apoptotic signal in normal cells and have been proposed to confer protection from TRAIL-inducing apoptosis. TRAIL may also play roles in immune responses, autoimmune disorders and HIV infection. TRAIL can interact with five different members of the TNF receptor superfamily, two of which contain a death domain, and can mediate an apoptotic response. TRAIL may also be a marker for progression of cancers.

for TRAIL antibody (mAb) (Clone 55B709.3)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TRAIL antibody (mAb) (Clone 55B709.3)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TRAIL antibody (mAb)
 

Data Thumbnails

TRAIL antibody (mAb) tested by Western blot.

Western blot of TRAIL mAb.
(Click image to enlarge and see details.)