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HDAC4 antibody (pAb)

Aliases: HD4, AHO3, BDMR, HDACA, HA6116, HDAC-4, HDAC-A
商品コード: 40969 Format: 100 µg ¥69,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:140 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for HDAC4 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µg per ChIP
ChIP-Seq: 5 - 10 µg each
WB: 1 - 2 µg/ml dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Published Applications:
ChIP
WB
See references for more information. Individual optimization may be required.

For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for HDAC4 antibody (pAb)Immunogen

This HDAC4 antibody was raised against a synthetic peptide corresponding to amino acid residues 194-209 of human HDAC4.

for HDAC4 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for HDAC4 antibody (pAb)References

 
HDAC4 antibody (pAb) tested by ChIP-Seq.

HDAC4 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from HeLa cells and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HDAC4 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

HDAC4 antibody (pAb) tested by Western blot.

HDAC4 antibody (pAb) tested by Western blot.
Detection of HDAC4 by Western blot. The analysis was performed using 293 nuclear extract and HDAC4 pAb. A protein band of approximate molecular weight of 140 kDa was detected.

for HDAC4 antibody (pAb)Background

HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

for HDAC4 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for HDAC4 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with HDAC4 antibody (pAb).

for HDAC4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HDAC4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HDAC4 antibody (pAb)
 

Data Thumbnails

HDAC4 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of HDAC4 pAb.
(Click image to enlarge and see details.)

HDAC4 antibody (pAb) tested by Western blot.

Western blot of HDAC4 pAb.
(Click image to enlarge and see details.)