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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | — |
for ATF-2 phospho Thr71 antibody (mAb)Background
ATF-2 (activating transcription factor 2) transcription factor is a member of the leucine-zipper family of proteins and was previously known as cAMP responsive element binding protein 2 (or CREB2). ATF 2 is a transcriptional activator that binds the cAMP-responsive element (CRE) and interacts with c-Jun to stimulate CRE transcription. This protein is also capable of acetylating Histone H2B and Histone H4 in vitro as a histone acetyltransferase (HAT).
for ATF-2 phospho Thr71 antibody (mAb)Immunogen
This ATF-2 phospho Thr71 antibody was raised against a synthetic peptide containing phosphorylated threonine at position 71 corresponding to human Phosphorylated-ATF-2.
for ATF-2 phospho Thr71 antibody (mAb)Application Notes
This ATF-2 phospho Thr71 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for ATF-2 phospho Thr71 antibody (mAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for ATF-2 phospho Thr71 antibody (mAb)Positive Control
Active Motif's HeLa nuclear extract (TNF-α stimulated) (Catalog No. 40210) can be used as a positive control for ATF-2 phospho Thr71 antibody.
ATF-2 phospho Thr71 mAb tested by Western blot.
ATF-2 (phospho) detection by Western blot. Detection of ATF-2 phosphorylation in HeLa cell extract with ATF-2 phospho Thr71 mAb.
Lane A: Untreated HeLa nuclear extract;
Lane B: HeLa nuclear extract treated with TNF-α for 1 hour.
for ATF-2 phospho Thr71 antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for ATF-2 phospho Thr71 antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting


