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Rad18 antibody (mAb)

Clone: 79B1048
Aliases: RNF73
商品コード: 40979 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Affinity Purified
Host:Mouse
Molecular Weight:60 kDa
Reactivity:Human

Applications

Western Blot Validated

for Rad18 antibody (mAb) (Clone 79B1048)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Rad18 antibody (mAb) (Clone 79B1048)Immunogen

This Rad18 antibody was raised against a synthetic peptide corresponding to amino acid residues 402-414 of human Rad18.

for Rad18 antibody (mAb) (Clone 79B1048)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Rad18 antibody (mAb) (Clone 79B1048)References

 
Rad18 antibody (mAb) tested by Western blot.

Rad18 mAb tested by Western blot.
Rad18 detection by Western blot. hRad18 mAb was diluted at 2 µg/ml (lane 1) and 1 µg/ml (lane 2) and tested against 10 µg of HL-60 nuclear extract by Western blot. A single band at 60 kDa is detected.

for Rad18 antibody (mAb) (Clone 79B1048)Background

Rad18 (RNF73, hHR18) is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Rad18 is similar to its yeast counterpart, as it is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif.

for Rad18 antibody (mAb) (Clone 79B1048)Positive Control

Active Motif's HL-60 nuclear extract (Catalog No. 36072) can be used as a positive control for Rad18 antibody.

for Rad18 antibody (mAb) (Clone 79B1048)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Rad18 antibody (mAb) (Clone 79B1048)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Rad18 antibody (mAb)
 

Data Thumbnails

Rad18 antibody (mAb) tested by Western blot.

Western blot of Rad18 mAb.
(Click image to enlarge and see details.)