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リコンビナントタンパク質・酵素

PvuRts1 I (PvuRts1I) restriction enzyme

商品コード: 55011 Format: 50 Units ¥47,000 Buy Now
Expressed In: E. coli Protein Species: Proteus vulgaris

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for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeContents

50 Units PvuRts1 I restriction enzyme
10X PvuRts1 I Reaction Buffer
1 M DTT

for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeApplication Notes

PvuRts1 I restriction enzyme can be used to directly cleave hydroxymethylated DNA in its non-glucosylated form. The PvuRts1 I enzyme also digests α and β-glucosylated hydroxymethylcytosine DNA, but at a lower efficiency than the non-glucosylated form. The enzyme is specific to 5-hmC DNA and will not digest 5-methylcytosine residues or unmethylated DNA. The enzyme is best suited for use in regions of high 5-hmC density, such as gene bodies.

for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeProtein Details

PvuRts1 I restriction enzyme recognizes the sequence hmCN11-12/N9-10G.

One unit is defined as the amount of enzyme required to fully cleave 1 µg of 5-hmC DNA Standard (Catalog No. 55008) in 30 minutes at 22°C in a total reaction volume of 30 µl. A pilot titration of enzyme is recommended for cleavage of genomic DNA.

 
PvuRts1 I restriction enzyme digestion of 5-hmC DNA.

PvuRts1 I restriction enzyme digestion of 5-hmC DNA shown by agarose gel.
One µg of unmethylated (Un), 5-methylcytosine (mC) or 5-hydroxymethylcytosine (hmC) Methylated DNA Standard (Catalog No. 55008) was incubated in the absence or presence of 1 unit PvuRts1 I enzyme for 30 minutes at 22°C. Each reaction was run on a 2.5% agarose gel alongside a 1 kb DNA ladder.

for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

PvuRts1 I (PvuRts1I) restriction enzyme
 

Data Thumbnails

PvuRts1 I restriction enzyme digestion of 5-hmC DNA.

Agarose gel of PvuRts1 I restriction enzyme digestion of 5-hmC DNA.
(Click image to enlarge and see details.)