Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

BRD8 / SMAP2 antibody (pAb)

Catalog No: 61007 Format: 100 µl $340 Buy Now
Catalog No: 61008 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Human
Molecular Weight:145 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:1,000

for BRD8 / SMAP2 antibody (pAb)Background

BRD8 (Bromodomain-containing protein 8, SMAP2, TRCP120) is a member of the Bromodomain-containing family of chromatin associated proteins. BRD8 is a member of the NuA4 histone acetyltransferase complex, involved in the transcriptional activation of specific genes through the acetylation of histones H2A and H4. BRD8 contains two Bromodomains, a motif that has been demonstrated to facilitate interaction with acetylated histones.

for BRD8 / SMAP2 antibody (pAb)Immunogen

This BRD8 / SMAP2 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human BRD8.

for BRD8 / SMAP2 antibody (pAb)Application Notes

This BRD8 / SMAP2 antibody has been validated for use in Western blotting (1:500 – 1:1,000).

for BRD8 / SMAP2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for BRD8 / SMAP2 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PRMT5 antibody.

 
BBRD8 / SMAP2 antibody (pAb) tested by Western blot.

Western blot of BRD8 / SMAP2 antibody.
Nuclear extract of HeLa cells (30 μg) probed with BRD8 / SMAP2 antibody (1:500).

for BRD8 / SMAP2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for BRD8 / SMAP2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

BRD8 / SMAP2 antibody (pAb)
 

Data Thumbnails

BBRD8 / SMAP2 antibody (pAb) tested by Western blot.

Western blot of BRD8 / SMAP2 antibody.
(Click image to enlarge and see details).