Your Cart
Browse
Filter
Histone H3 dimethyl Lys36 antibody (mAb)
| Clone: MABI 0332 |
| Catalog No: 61019 | Format: 100 µg | $340 | Buy Now |
|
|
Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 0.5 - 2 µg/ml | 2 - 5 µg per ChIP | 0.5 - 1 µg/ml | 1 - 5 µg per IP |
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)Immunogen
This Histone H3 dimethyl Lys36 antibody was raised against a peptide containing dimethyl Lys36 of human Histone H3.
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)Application Notes
This Histone H3 dimethyl Lys36 antibody chromatin IP (ChIP) (2 - 5 µg per ChIP), immunofluorescence (0.5 - 1 µg/ml), immunoprecipitation (1 - 5 µg per IP) and Western blotting (0.5 - 2 µg/ml). See references for more details.
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 dimethyl Lys36 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 dimethyl Lys36 antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3 dimethyl Lys36 antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 dimethyl Lys36 antibody (mAb).
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)References
The creation of this antibody was described in Kimura, H. et al (2008) Cell Struct Func 33: 61-73.
This antibody has been used in the following references:
ChIP: Rechtsteiner A et al (2010) PLoS Genet 6: e1001091.
Western blot of Histone H3 dimethyl Lys36 antibody.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 dimethyl Lys36 antibody (2 µg/ml dilution).
Dot blot of Histone H3 dimethyl Lys36 antibody.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys36 antibody for dimethyl Lys36 of histone H3. Recombinant methylated peptides corresponding to the immunogen and related sequences were spotted onto PVDF and probed with Histone H3 dimethyl Lys36 at 2 µg/ml. The amount of protein (picomoles) spotted is indicated next to each row.
Top panel - Lane 1: unmodified H3 Lys4 peptide. Lane 2: monomethyl Lys4 peptide. Lane 3: dimethyl Lys4 peptide. Lane 4: trimethyl Lys4 peptide. Lane 5: unmodified Lys9 peptide. Lane 6: monomethyl Lys9 peptide. Lane 7: dimethyl Lys9 peptide. Lane 8: trimethyl Lys9 peptide. Lane 9: unmodified Lys79 peptide. Lane 10: monomethyl Lys79 peptide. Lane 11: dimethyl Lys79 peptide. Lane 12: trimethyl Lys79 peptide.
Bottom panel - Lane 1: unmodified H3 Lys23 peptide. Lane 2: monomethyl Lys23 peptide. Lane 3: dimethyl Lys23 peptide. Lane 4: trimethyl Lys23 peptide. Lane 5: unmodified Lys27 peptide. Lane 6: monomethyl Lys27 peptide. Lane 7: dimethyl Lys27 peptide. Lane 8: trimethyl Lys27 peptide. Lane 9: unmodified Lys36 peptide. Lane 10: monomethyl Lys36 peptide. Lane 11: dimethyl Lys36 peptide. Lane 12: trimethyl Lys36 peptide.
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 dimethyl Lys36 antibody (Clone mAb) (Clone MABI 0332)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
