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Progesterone Receptor antibody (mAb)

Clone: 6A
Aliases: PGR, PR, NR3C3
商品コード: 61023 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:100 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for Progesterone Receptor antibody (mAb) (Clone 6A)Application Notes


Validated Applications:
ChIP: 0.5 - 2 µg/ml dilution

Published Applications:
ICC/IF
IHC
WB
See references for more information. Individual optimization may be required.

for Progesterone Receptor antibody (mAb) (Clone 6A)Immunogen

This Progesterone Receptor antibody was raised against a peptide within the C-terminus of human Progesterone receptor.

for Progesterone Receptor antibody (mAb) (Clone 6A)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Progesterone Receptor antibody (mAb) (Clone 6A)References

 
Progesterone Receptor antibody (mAb) tested by ChIP.

Progesterone Receptor antibody (mAb) tested by ChIP.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 4.5 million treated MCF-7 breast cancer cells and 4 µl of Progesterone Receptor antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme, which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

for Progesterone Receptor antibody (mAb) (Clone 6A)Background

Progesterone Receptor (PR, NR3C3) is a nuclear receptor that binds the steroid hormone progesterone, translocates into the nucleus and activates transcription.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Progesterone Receptor antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Progesterone Receptor antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Progesterone Receptor antibody (mAb).

for Progesterone Receptor antibody (mAb) (Clone 6A)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Progesterone Receptor antibody (mAb) (Clone 6A)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Progesterone Receptor antibody (mAb)
 

Data Thumbnails

Progesterone Receptor antibody (mAb) tested by ChIP.

ChIP of Progesterone Receptor mAb.
(Click image to enlarge and see details.)