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PPAR-γ antibody (mAb)

Clone: 3PP-1H4
Aliases: GLM1, CIMT1, NR1C3, PPARG1, PPARG2
商品コード: 61025 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:55 kDa
Reactivity:Human

Applications

Western Blot Validated

for PPAR-g antibody (mAb) (Clone 3PP-1H4)Application Notes


Published Applications:
WB
See references for more information. Individual optimization may be required.

for PPAR-g antibody (mAb) (Clone 3PP-1H4)Immunogen

This PPAR-gamma antibody was raised against a peptide within the N-terminal region of human PPAR gamma 1 and gamma 2.

for PPAR-g antibody (mAb) (Clone 3PP-1H4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PPAR-g antibody (mAb) (Clone 3PP-1H4)References

for PPAR-g antibody (mAb) (Clone 3PP-1H4)Background

Peroxisome Proliferator-Activated Receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: α, δ and γ. PPAR-γ stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs bind to peroxisome proliferator response elements (PPREs) as heterodimers with the retinoid X receptor (RXR).

for PPAR-g antibody (mAb) (Clone 3PP-1H4)Positive Control

Active Motif's THP-1 nuclear extract (Catalog No. 36076) can be used as a positive control for PPAR-γ antibody.

for PPAR-g antibody (mAb) (Clone 3PP-1H4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PPAR-g antibody (mAb) (Clone 3PP-1H4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PPAR-γ antibody (mAb)