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Antibodies

RAR-β antibody (mAb)

Clone: 8B-10B2
Catalog No: 61027 Format: 100 µg $340 Buy Now
Catalog No: 61028 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein G Chromatography
Applications:EMSA, IHC, IP, WB
Reactivity:Human
Molecular Weight:50 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
0.5 - 2 µg/ml — Published.
See Application Notes below.

for RAR-beta antibody (mAb) (Clone 8B-10B2)Background

RAR-β (HAP, NR1B2) is a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. RAR-β localizes to the cytoplasm and to subnuclear compartments. RAR-β binds retinoic acid, the biologically active form of vitamin A which mediates cellular signaling in embryonic morphogenesis, cell growth and differentiation. It is thought that RAR-β limits growth of many cell types by regulating gene expression.

for RAR-beta antibody (mAb) (Clone 8B-10B2)Immunogen

This RAR-beta antibody was raised against amino acids 413-448 of human RAR-beta.

for RAR-beta antibody (mAb) (Clone 8B-10B2)Application Notes

This RAR-β antibody has been validated for use in EMSA, immunohistochemistry (1 - 4 µg/ml dilution), immunoprecipitation and Western blotting (0.5 - 2 µg/ml dilution). See references for more details.

for RAR-beta antibody (mAb) (Clone 8B-10B2)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for RAR-beta antibody (mAb) (Clone 8B-10B2)Positive Control

Active Motif's Recombinant RAR-β protein (Catalog No. 31131) can be used as a positive control for RAR-β antibody.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use RAR-β antibody (mAb).

for RAR-beta antibody (mAb) (Clone 8B-10B2)References

This antibody has been used in the following references:
EMSA: Wan H et al (1999) Cancer Res 61: 556-564.
IHC: Boehm N et al (2004) Eur J Dermatol 14: 19-23; Chakravarti N et al (2001) Int J Cancer 91: 27-31.
WB: Wan H et al (1999) Cancer Res 61: 556-564.

for RAR-beta antibody (mAb) (Clone 8B-10B2)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for RAR-beta antibody (mAb) (Clone 8B-10B2)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting