Histone H2B antibody (mAb)

for Histone H2B antibody (mAb) (Clone 5HH2-2A8)Background

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H2B antibody (mAb) (Clone 5HH2-2A8)Immunogen

This histone H2B antibody was raised against a peptide from the C-terminal region of human Histone H2B.

for Histone H2B antibody (mAb) (Clone 5HH2-2A8)Application Notes

This histone H2B antibody has been validated for use in Western blot (0.1 - 1 µg/ml dilution). See references for more details.

for Histone H2B antibody (mAb) (Clone 5HH2-2A8)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Histone H2B antibody (mAb) (Clone 5HH2-2A8)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H2B antibody.

for Histone H2B antibody (mAb) (Clone 5HH2-2A8)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H2B antibody (mAb) (Clone 5HH2-2A8)Guarantee

This product is guaranteed for 6 months from date of arrival.