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Antibodies

CDT1 antibody (mAb)

Catalog No: 61039 Format: 100 µg $340 Buy Now
Catalog No: 61040 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Hamster
Isotype:IgG
Purification:Protein G Chromatography
Applications:IHC, IP, WB
Reactivity:Human
Molecular Weight:65 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
0.5 - 2 µg/ml — 2 - 10 µg per IP

for CDT1 antibody (mAb)Background

CDT1 (chromatin licensing and DNA replication factor 1) is a key licensing factor which functions to allow DNA replication. It is recruited (along with CDC6) by the ORC (Origin Recognition Complex) to sites of DNA replication and helps form the pre-replicative complex (pre-RC). Its activity during the cell cycle is regulated by its association with the Geminin protein geminin, which both inhibits CDT1 activity during S phase in order to prevent unregulated DNA replication and also to inhibit CDT1 proteolysis.

for CDT1 antibody (mAb)Immunogen

This CDT1 antibody was raised against a recombinant protein corresponding to amino acids 177-557 of mouse CDT1.

for CDT1 antibody (mAb)Application Notes

This CDT1 antibody has been validated for use in immunohistochemistry, immunoprecipitation (2-10 µg per IP) and Western blotting (0.5 - 2 µg/ml dilution). See references for more details.

for CDT1 antibody (mAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for CDT1 antibody (mAb)References

This antibody has been used in the following references:
WB: Arentson E et al (2002) Oncogene 21: 1150-1158.

for CDT1 antibody (mAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for CDT1 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting

Technical Data Sheet

CDT1 antibody (mAb)