HMGA2 / HMGI-C antibody (pAb)

RRID: AB_2614960
Aliases: BABL, LIPO, HMGIC, STQTL9
Catalog No: 61041 Format: 100 µl ¥86,000 Buy
Catalog No: 61042 Format: 10 µl ¥24,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
20 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes

Applications Validated by Active Motif:
IP: 10 µl per IP
WB*: 1:1,000 - 1:5,000 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This HMGA2 antibody was raised against a peptide within the N-terminal region of human HMGA2 / HMGI-C.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

HMGA2 / HMGI-C antibody (pAb) tested by Immunoprecipitation.
10 µl of HMGA2 / HMGI-C antibody was used to immunoprecipitate HMGA2 / HMGI-C from 250 µg of HepG2 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the HMGA2 / HMGI-C antibody at a dilution of 1:3,000.

HMGA2 / HMGI-C antibody (pAb) tested by Western blot.
Detection of HMGA2 / HMGI-C by Western blot. Nuclear extract (25 µg) of mouse ES cells probed with HMGI-C / HMGA2 antibody at a dilution of 1:3,000.

Background

HMGA2 (High-mobility group AT-hook protein 2, HMGI-C) is a member of the HMG (High mobility group) protein family, which are non-histone chromatin-associated proteins involved in DNA organization and regulation of transcription. The HMG protein family consists of six proteins and is subdivided into three subfamilies: the HMG-1/-2 subfamily, the HMG-I/Y subfamily and the HMG-14/-17 subfamily. Mouse models of HMGA2 function indicate that it is involved in proliferation at the cellular level and obesity at the organismal level. Overexpression of HMGA2 is observed in a variety of cancers.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting