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Antibodies

HMGA2 / HMGI-C antibody (pAb)

Catalog No: 61041 Format: 100 µl $340 Buy Now
Catalog No: 61042 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Human
Molecular Weight:20 kDa

Applications Table

Western Blot Chromatin IP
1:1,000 - 1:5,000

for HMGA2 / HMGI-C antibody (pAb)Background

HMGA2 (High-mobility group AT-hook protein 2, HMGI-C) is a member of the HMG (High mobility group) protein family, which are non-histone chromatin-associated proteins involved in DNA organization and regulation of transcription. The HMG protein family consists of six proteins and is subdivided into three subfamilies: the HMG-1/-2 subfamily, the HMG-I/Y subfamily and the HMG-14/-17 subfamily. Mouse models of HMGA2 function indicate that it is involved in proliferation at the cellular level and obesity at the organismal level. Overexpression of HMGA2 is observed in a variety of cancers.

for HMGA2 / HMGI-C antibody (pAb)Immunogen

This histone HMGA2 antibody was raised against a peptide within the N-terminal region of human HMGA2 / HMGI-C.

for HMGA2 / HMGI-C antibody (pAb)Application Notes

This HMGA2 antibody has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution).

for HMGA2 / HMGI-C antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

 
HMGA2 / HMGI-C pAb tested by Western blot.

HMGA2 pAb tested by Western blot.
Detection of HMGA2 / HMGI-C by Western blot. Nuclear extract (25 µg) of mouse ES cells probed with HMGI-C / HMGA2 antibody at a dilution of 1:3,000.

for HMGA2 / HMGI-C antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HMGA2 / HMGI-C antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

HMGA2 / HMGI-C antibody (pAb)
 

Data Thumbnails

HMGA2 / HMGI-C pAb tested by Western blot.

Western blot of Histone HMGA2 / HMGI-C pAb.
(Click image to enlarge and see details).